CIPA

News:

NMI one out of two core sites in CiPA Myocyte study

We are very excited that HESI changed our status from non-core to core site for the actual running CiPA myocyte study.

The mission of our electrophysiology group in this study is to test the (pro-)arrhythmic potential of 28 compounds (blinded to the participants) on hiPSC-derived cardiomyocytes from both Axiogenesis and Cellular Dynamics using the 96-well version of the Multiwell MEA system by Multi Channel Systems. Together with the data obtained by the other core sites this data will be used to assess the usability of hiPSC-derived cardiomyocytes on the MEA system.

Additionally we participate in testing 12 blinded compounds on hiPSC-cardiomyocytes from Takara/Cellartis and Pluriomics, who generously sponsor CiPA and us with their cells.

As member of HESI the NMI already successfully participated in the CiPA Myocyte pilot study in 2014/2015.

CiPA (the Comprehensive in Vitro Proarrhythmia Assay initiative) is a project driven by the Health and Environmental Science Institute (HESI), the FDA and the Safety Pharmacology Society. It aims to optimize the assessment of clinical potential of TdP and to replace the preclinical hERG current assay required under the ICH S7B safety pharmacology guideline.

For more information visit www.cipaproject.org

We offer a complete range of services related to CIPA

Cardiomyocyte Services:

Drug-induced effects on cardiac function can be observed in our test system, as a prolongation of the fAP duration (“QT-interval in vitro”) and/or arrhythmic beating behaviour of the electrically coupled cardiomyocytes.

Cardiomyocytes after DIV 3 forming a syncytium of electrically coupled cells	Field action potentials (fAPs) recorded from cardiomyocytes at DIV3 representing the "QT interval
Effect of quinidine on "QT-interval" in vitro	Dose-response curve for cisapride action on "QT-interval" in vitro
Drug-induced arrhythmia in cardiomyocytes (DIV 3 on MEA)

Ion Channel Services:

We use the industry leading platforms Qpatch and Patchliner for ion channel screening. Our cardiac panel of ion channels include:

hERG
NaV1.5
CaV1.2 (new cell line)
CaV3.2
KvLQT1/minK
KV4.3
Kir2.1
KV1.5